Here, we provide a detailed protocol for the single protein production (SPP) system, which is designed to produce only a single protein of interest in living Escherichia coli cells. Induction of MazF, ...
Pure Production and Stable Isotopic Labeling of Desired Proteins with the SPP System Elucidation of protein structure and function maintains an important role in post-genomic sequencing and analysis ...
Cold shock–inducible vectors enable the production of labeled proteins in bacteria for structural and functional studies. You have full access to this article via your institution. Previous tests of ...
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