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jove.com
Video: Restriction Enzyme Digests: Principle, Procedure, and Applications
299.3K Views. Restriction enzymes or endonucleases recognize and cut DNA at a specific sequence. These enzymes occur naturally in bacteria as a defense against bacteriophages - viruses that infect bacteria. Bacterial restriction enzymes cut the invading bacteriophage DNA while leaving the bacterial genomic DNA unharmed due to addition of methyl ...
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Feb 1, 2013
Restriction enzyme DNA Cutting
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14K views · 446 reactions | EcoRI cutting a strand of DNA. EcoRI is a restriction endonuclease that recognizes and cuts DNA at a specific motif (GAATTC). After the DNA is cut, so-called sticky ends are left behind. Sticky ends are short non-paired DNA bases. This property makes this enzyme a powerful tool in molecular biology. The enzyme naturally occurs in E. coli, where it serves as a defense mechanism against viral DNA. | Ribosome Studio | Facebook
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Ribosome Studio
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